Article Text


Cerebral oxygenation during cardiopulmonary bypass
  1. S P Wardle,
  2. C W Yoxall,
  3. A M Weindling
  1. Department of Child Health, Royal Liverpool Children’s Hospital
  1. Dr S P Wardle, Department of Child Health, University of Liverpool, Neonatal Unit, Liverpool Women’s Hospital , Crown Street, Liverpool L8 7SS. e-mail: s.p.wardle{at}


Cerebral fractional oxygen extraction (FOE) was monitored in 30 children, using near infrared spectroscopy during cardiopulmonary bypass, to investigate the effect of hypothermia and circulatory arrest. One group of children (n = 15) underwent profound hypothermia with total circulatory arrest (n = 8) or continuous flow (n = 7). Another group (n = 15), of whom only one had circulatory arrest, underwent mild (n = 6) or moderate (n = 9) hypothermia.

 The mean FOE (SD) before bypass was 0.35 (0.12) and this correlated negatively with the preoperative arterial oxygen content (r=−0.58). Between the stage of cooling on bypass and cold bypass there was a reduction in FOE in all groups. Between cold bypass and rewarming there was an increase in FOE only in the groups with continuous flow. In the circulatory arrest group, the FOE remained low during rewarming and was significantly lower than that of the continuous flow group. No patients died and none had neurological abnormalities postoperatively.

 Apparent changes in oxidised cytochrome oxidase concentration were also monitored using near infrared spectroscopy. There was a fall in cytochrome aa3 on starting cardiopulmonary bypass, but there were no significant differences in the changes in cytochromeaa3 between any stage in any of the patient groups.

 Using this non-invasive technique, cooling was shown to reduce cerebral FOE. During rewarming on bypass there was an increase in cerebral FOE only in patients who had had continuous flow bypass. In contrast, the cerebral FOE in those with circulatory arrest remained constant after arrest and during the duration of the study. This may have implications for the timing of hypoxic brain injury.

  • fractional oxygen extraction
  • hypothermia
  • circulatory arrest
  • near infrared spectroscopy

Statistics from

Hypothermia and cardiopulmonary bypass are widely used techniques in paediatric cardiac surgery. Brain injury is a serious cause of morbidity after these procedures and has been reported as occurring in between 1–25% of paediatric operations.1Hypothermia is used for brain protection mainly because it reduces tissue metabolism and may cause a decrease in free radical activity.2-4 It may also be useful in other situations where cerebral protection is needed, for example after birth asphyxia.

It has not previously been possible to measure cerebral venous saturation (Svo 2) and fractional oxygen extraction (FOE) non-invasively during cardiopulmonary bypass. Previous studies using invasive methodologies have shown changes in cerebral oxygenation during cardiac bypass,5-7 the most important being a decrease in oxygen consumption during hypothermia and a fall in cerebral venous saturation during rewarming. Non-invasive studies by near infrared spectroscopy during hypothermia and circulatory arrest of changes in the relative concentrations of the chromophores oxyhaemoglobin and deoxyhaemoglobin and oxidised cytochrome oxidase have relied on estimates of the path length of light to produce continuous recordings of semiquantitative measurements of the concentrations of the chromophores to study trends in the change in oxygenation in the brain.7-12 Absolute quantification, however, has not been possible. Absolute measurements of cerebral Svo 2 can, however, be made using near infrared spectroscopy, without reference to an optical path length estimation, by causing a brief partial jugular venous occlusion.13This technique has been validated by comparison with invasive measurements of cerebal Svo 2 from co-oximetry of blood from the jugular bulb.13 Measurements of cerebral Svo 2, together with measurements of arterial saturation (Sao 2)made by pulse oximetry, can be used to calculate the cerebral FOE, a measure of the balance between the oxygen consumption and oxygen delivery.

FOE varies from organ to organ and with levels of activity. Measurements of FOE for the whole body produce a range of approximately 0.15 to 0.33. That is the body consumes 15% to 33% of oxygen transported.14 15 The heart and brain are likely to have consistently high values of FOE, whereas muscle may have variable levels with higher oxygen extraction during active states.

The purpose of this study was to investigate by a non-invasive method cerebral oxygenation during induced hypothermia. Our primary hypothesis was that cooling for cardiac bypass operations reduced cerebral FOE.

Near infrared spectroscopy may also be able to monitor the redox status of cytochrome oxidase (cytochrome aa3), the terminal enzyme in the respiratory electron transport chain responsible for ATP production within the cell. In hypoxic piglets, reduction of cytochromeaa3 has been shown to correlate with a decrease in brain energy state measured by nuclear magnetic resonance spectroscopy.16 Monitoring this variable might therefore provide useful information on the intracellular use of oxygen. A secondary hypothesis of this study was that there was a difference in the changes in cerebral FOE and cytochrome aa3 in children subjected to circulatory arrest during hypothermia.



Measurements were made using the Hamamatsu NIRO-500 (Hamamatsu UK). Data were stored in a computer file for analysis offline, using a spreadsheet package (Lotus 1-2-3).

After induction of anaesthesia, the monitoring optodes were positioned on the frontotemporal region of the child’s head. One optode was placed centrally on the forehead below the hairline, and the other was positioned temporally as far away as possible, on the forehead and below the hairline. In practice, the interoptode distance was between 4–7 cm, the exact distance depending on the size and shape of the child’s head. The optodes were fixed tightly in place using elastic adhesive tape, with the aim of reducing scalp blood flow in the optical field. The monitoring optodes were removed before the patient’s transfer to the paediatric intensive care unit.

Using measurements of cerebral Svo 2 by near intrared spectroscopy with partial venous occlusion and Sao 2 by pulse oximetry, values for the cerebral FOE were calculated: FOE = (Sao 2−Svo 2) /Sao 2.

These measurements involved minimal disturbance of the subject, and repeated measurements were possible. It was not possible to make measurements of FOE during circulatory arrest, as measurement of FOE by this method requires continuous circulation.

Each measurement of Svo 2 involved making five unilateral partial jugular venous occlusions which took approximately 30 seconds. The Svo 2 was calculated as the mean of these five measurements. They were made every 10 minutes throughout the operation, most frequently during cooling and rewarming. Measurements of Sao 2, arterial blood pressure, and nasal temperature (representing brain temperature) were recorded at the time of each measurement of cerebral Svo 2. In addition, the packed cell volume was measured approximately every 30 minutes at each stage of the operation.

At the time of each measurement of cerebral FOE, cytochromeaa3 data was logged every 0.5 seconds, and these data were averaged over a 60 second period. These measurements were made throughout the operation including during hypothermic circulatory arrest. The differential path length factor was set at that recommended for the neonatal head in children less than 3 months of age, and set for that recommended for the adult head in the other children.17


Hypothermia was graded according to the minimum core temperature achieved during cooling. During paediatric operations at this hospital, children are cooled to target temperatures of 15°C (profound hypothermia), 22–24°C (moderate hypothermia), or 28°C (mild hypothermia), and circulatory arrest is used in selected children according to the surgical requirements.

A central venous catheter was inserted and a femoral artery cannulated for monitoring of systemic arterial pressure. A urinary catheter and nasopharyngeal and oesophageal temperature probes were inserted after induction of anaesthesia and a peripheral tissue oxygen saturation probe was taped in place.

The haemoglobin concentration was measured the day before the operation and the arterial oxygen content (Cao2) was calculated using this and the Sao 2 at the time of measurement of the cerebral FOE before bypass: Cao2=(haemoglobin)×1.34×SaO2, where 1.34 is the quantity of oxygen (ml) carried by 1 g of haemoglobin, and assumes that the quantity of dissolved oxygen is negligible.

A standard anaesthetic protocol was used for each of the patients and the degree and duration of hypothermia was decided by the surgeon. The hollow fibre D701 oxygenator (Dideco) used for cardiopulmonary bypass was primed with warmed blood and crystalloid. Bypass flow was set initially at 2.4 l/min/m2 and this was altered in some patients to suit surgical requirements. The rate of cooling and rewarming was controlled to approximately 1°C per minute. During surgical preparation patients were surface cooled by exposure to a 15°C ambient operating theatre. The packed cell volume was maintained at 25+/−5%. Intermittent arterial blood gas measurement was used to maintain a constant arterial partial pressure of oxygen (Pao2) aided by the use of an inline Pao2monitor (Polystan). Similarly, a capnograph (Datex Ltd) attached to the gas outlet port of the oxygenator helped to maintain a constant arterial carbon dixoide tension (5.3 +/− 0.5 kPa, temperature uncorrected, that is, alpha-stat regulation).18 Inotropic support was given as required to aid weaning from cardiopulmonary bypass during rewarming.


This was an observational study. Thirty children undergoing operations involving hypothermia were studied after informed parental consent. Measurements were made on 30 children. Fifteen children underwent profound hypothermia and eight of these underwent total circulatory arrest; nine children underwent moderate hypothermia of whom one had circulatory arrest. None of the children who had mild hypothermia (n = 6) underwent circulatory arrest.

The neurological status of the children was assessed daily during the first seven postoperative days by clinical neurological examination. Abnormal neurological status was defined as focal or generalised abnormal neurological signs, unexpected decreased consciousness that was not attributable to sedative drugs, or seizures.

The operation was divided into five arbitrary stages: before bypass, cooling on bypass, cold bypass, rewarming on bypass, and after bypass. Before bypass was defined as the period between the first measurement made after the patient was stable after induction of anaesthesia and before bypass flow began. Cooling was the period during which the patient was being actively cooled on cardiopulmonary bypass with full bypass flow. Cold bypass was the period during which the minimum core temperature was reached and during which the temperature did not vary by more than +/− 1°C; during this period some patients were subjected to total circulatory arrest and alterations in bypass flow rate. Rewarming bypass was the period between the start of active rewarming on bypass and cessation of bypass flow. After bypass was the period between cessation of bypass flow and the end of the operation.


The mean FOE for each patient was determined for each stage. Patients were then considered as groups: cerebral FOE of children with profound hypothermia were compared with those who had mild/moderate hypothermia; the effect of circulatory arrest was compared with that of continuous flow bypass. Changes in mean FOE and median cytochromeaa3 between stages of the operation in the different groups under consideration were analysed using analysis of variance (ANOVA) and paired t tests for changes in mean FOE at each stage, and ANOVA and Wilcoxon rank sum tests for changes in the cytochrome aa3. Unpaired t tests were used to assess differences in mean FOE among groups.


Table 1 summarises the patient details in the groups studied. None of the children in the study died or had any postoperative neurological abnormalities detected.

Table 1

 Descriptive details of patients studied. Variables are shown as medians and ranges

The median (range) number of measurements was 5 (1–18) during each stage of the operation, depending on the length of each stage. Six children did not have FOE measurements taken during cold bypass because it was not possible in the time available, or because circulatory arrest was started as soon as the minimum temperature was reached.

The bypass flow rate was altered in six children during cold bypass other than for episodes of circulatory arrest. Bypass flow was reduced to half for a median time of 16 minutes (range four to 60 minutes). Only one patient was reduced to quarter flow for a 15 minute period. There was no relation between flow rate and FOE within individuals (r = 0.09, p = 0.31 ) during cold bypass and there was no difference in mean FOE at any stage of the operation among those with reduced flow rate and those with full flow rate (p = 85, p = 0.62 for cold bypass and rewarming respectively). Table 2 summarises the changes in blood pressure, temperature, and haemoglobin concentration in the different groups.

Table 2

Summary of changes in blood pressure, nasal temperature, haemoglobin concentration, cerebral FOE, and oxidised cytochrome oxidase concentration in the different groups. FOE is expressed as mean (SD), other variables are expressed as median (range)


Before cardiac bypass, the mean FOE (SD) was 0.360 (0.12). The mean FOE (SD) at the end of the operation was 0.384 (0.12), and this was not significantly different to the before bypass value (n = 30, p = 0.23).

The mean (SD) preoperative haemoglobin concentration was 13.1 (2.3) g/dl. The mean FOE before bypass correlated negatively with the preoperative haemoglobin concentration (n = 30, r = −0.637, p < 0.01) and with the arterial oxygen content (n = 30,r = −0.58, p = 0.001) (fig 1). The temperature and systolic blood pressure showed little variation during this stage as the patients were stable and anaesthetised and not being actively cooled.

Figure 1

Relation between preoperative arterial oxygen content and mean cerebral FOE before cardiac bypass (n = 30, r = −0.58, p = 0.001).

During the subsequent stages, nine (30%) of the patients had at least one FOE measurement operation above the 95th centile (0.60) for the preoperative normal range. Six children had an FOE above the 95th centile on more than one occasion: in five (55.6%), these episodes occurred during rewarming, and in five (55.6%), they occurred during the period after bypass. Since measurements were made intermittently, it was not possible to determine exactly how long these episodes lasted. In eight cases the total period during which FOE was increased above the 95th centile was less than 10 minutes, but in one case there was a 20 minute period where the cerebral FOE was persistently above 0.60. This was during the rewarming period in a child subjected to profound hypothermia with continuous flow bypass, and whose short term outcome was normal. None of the children had any signs of neurological injury.


The results from children who underwent mild or moderate hypothermia were considered together because the changes in these two groups were similar. There were 15 children in the profound hypothermia group and 15 children in the mild/moderate hypothermia group. Table 3shows the mean FOE during each stage of the bypass procedure. There were no significant differences between these two groups at any stage of the operation.

Table 3

Results of analysis of changes in mean FOE (SE) between each stage of the operation for the different temperature groups


Eight of the children in the profound hypothermia group, and one in the moderate hypothermia group, were subjected to total circulatory arrest. They were compared with the 21 other children studied where continuous flow bypass was used. The median (range) arrest time was 32 (14–64) minutes. In the circulatory arrest group, the mean FOE (SD) during cold bypass before the onset of circulatory arrest was 0.392 (0.11) and there was no significant change in mean FOE (SD) on rewarming (n = 7, p = 0.22). The mean FOE (SD) in the continuous flow group during cold bypass was 0.362 (0.16) and in this group there was a significant increase during rewarming (n = 17, p = 0.013, see fig 2). The mean FOE (SD) during rewarming of children who underwent circulatory arrest was 0.352 (0.08), and this was significantly lower (p = 0.006) for those cases in which there was no circulatory arrest (0.452 (0.09), fig 2). This was not related to the temperature when circulatory arrest occurred or the duration of cold bypass. In the after bypass period the mean FOE (SD) of the continuous flow group was 0.395 (0.13); this was a significant decrease (n = 21, p = 0.03). By contrast the FOE of the circulatory arrest group remained low after bypass at 0.360 (0.12) and this was not significantly different than the FOE during rewarming (n = 9, p = 0.86, fig 2). When comparing the two groups there was no significant difference in cerebral FOE in the period after bypass (p = 0.5).

Figure 2

Changes in cerebral FOE (mean +/− standard error) in circulatory arrest and continuous flow groups during the five stages of bypass operation. * p = 0.006 unpaired t test between circulatory arrest and continuous flow groups. The other stages showed no sifnificant difference between the groups. See text for further comparisons.


There were eight patients who were cyanosed preoperatively (Sao 2 < 90%). The FOE of these patients was significantly lower than that of patients who were not cyanosed. Their haemoglobin concentrations were also significantly higher (table 4). When these patients were started on bypass the oxygen saturation increased significantly and there was a significant rise in FOE (table4). In the patients who were not cyanosed there was no significant change in FOE at the start of bypass compared with the level before bypass. On bypass, the FOE of the patients with cyanotic heart disease (0.388 (0.16)) was similar to those who were not (0.410 (0.10), p=0.67). Table 4 shows the changes in haemoglobin concentration, Sao 2, mean FOE in cyanosed and non-cyanosed children.

Table 4

Haemoglobin concentration (mean (SD)), Sao 2, (median (IQR)), FOE (mean (SD)), and arterial oxygen content (mean (SD)) in cyanosed and non-cyanosed patients at the start of bypass


In the whole group, there was a significant fall in cytochromeaa3 from before bypass to cooling. The median change (interquartile range, IQR) was −0.66 μmol/l (−1.08 to −0.20) (n = 30, p = 0.03). There was no significant change between the other stages. For each individual there was no significant relation between the cerebral FOE and change in cytochrome aa3 (r = 0.05, p = 0.25). The change in median cytochromeaa3 (IQR) between the before bypass and after bypass stages was 0.59 umol/l (0.16 to 1.44). This change was not statistically significant (p = 0.74).

At the start of bypass, there was a significant decrease in cytochromeaa3 in those who were not cyanosed (p = 0.001, n = 22), but no significant change in those who were cyanosed (p = 0.5, n = 8) (table 4). Table 4 summarises the changes in oxidised cytochrome oxidase concentration.


We have shown that during rewarming after cardiac bypass, the cerebral FOE of children who underwent profound hypothermic circulatory arrest remained low. This was in contrast with the results from patients who underwent profound hypothermia and continuous flow in whom cerebral FOE rose significantly. There are two possible explanations. One is that profound hypothermic circulatory arrest induced a decrease in cerebral metabolic rate which persisted in spite of rewarming.7 This observation is in line with those from studies using a piglet model of profound hypothermic circulatory arrest: cerebral metabolism was still depressed at 45 minutes of reperfusion but returned to normal only after three hours of reperfusion.19 Another possibility is that cerebral cellular function may have been altered in the course of circulatory arrest with inefficient oxygen extraction and a low observed FOE. In this situation, cerebral lactate production would be expected to rise, and this has been shown by van der Linden et al who suggested that global ischaemia during circulatory arrest impeded optimal aerobic glucose metabolism.20

None of the children in this study developed neurological problems, and so presumably none exceeded the ‘safe’ range for cerebral FOE either in terms of duration or absolute value. This was in spite of the fact that an increase above the 95th centile for FOE was seen in more than a quarter of the patients studied. This observation is in line with other studies which studied changes in Svo2 invasively. These studies also reported frequent episodes of “cerebral desaturation” during bypass and found that these episodes were not associated with postoperative neurological impairment.21 22 However, one study by Croughwellet al related low concentrations of Svo 2 measured during cardiac surgery in adults with an impaired postoperative cognitive performance suggesting that global hypoperfusion may have been a cause of this problem after surgery.2

The cerebral FOE before bypass was strongly related to the preoperative arterial oxygen content. During this stage of the operation, the patient was relatively stable without fluctuations in cardiac output, blood gases, blood volume, or temperature, so that other factors affecting oxygen delivery were relatively constant. All were unconscious due to anaesthesia, so presumably cerebral oxygen demands were also relatively constant.

In patients in whom continuous flow bypass was used, a significant rise in FOE occurred during rewarming on bypass. This suggested that there had been a change in the balance between supply and consumption of oxygen, either because the cerebral metabolic rate for oxygen had increased at this stage or oxygen delivery had fallen. This stage is therefore likely to be the time when the balance between oxygen delivery and consumption could become compromised in those with continuous flow and therefore when patients may be most vulnerable to hypoxic brain injury.

Considering all 30 patients, FOE decreased between the stage of cooling and cold bypass. Not all patients who underwent mild/moderate hypothermia showed a decrease in FOE between cooling and cold bypass. The decrease in FOE was only significant in those subjected to profound hypothermia. Sao 2 did not change during bypass, and the decrease in FOE was due to an increase in Svo 2. Other studies which have measured Svo 2 by invasive methods have also shown an increase during cooling.5-7 A drop in temperature was the only major physiological change which occurred between these stages. It therefore seems reasonable to assume that the observed decrease in FOE was due to hypothermia and was caused by an increase in Svo 2, which is likely to be as a result of a fall in cerebral oxygen consumption.

In patients who had cyanotic heart disease, there was a significant rise in FOE when bypass was started, but there was no observed change in FOE in patients who were not cyanosed. The explanation for this difference is likely to be due to the interaction of physiological changes which occur when bypass starts. The oxygen saturation increases as blood oxygenation is taken over by the bypass machine, but there is also haemodilution in the bypass circuit. In addition, as the temperature falls, metabolic rate also falls and oxygen demand would be expected to decrease. The direction of change in FOE will reflect the balance of these processes.

Because cytochrome aa3 changes are difficult to validate and may be affected by changes in brain temperature and cerebral water content during the course of measurement, the significance and validity of our observations of changes in cytochrome aa3 are not known. Because of the variation in path length among individuals, comparisons of cytochrome aa3 among groups of patients were not possible. No significant difference in cytochromeaa3 was shown during rewarming in those undergoing circulatory arrest in our study despite the difference in FOE, and no change in cytochrome aa3 was seen during arrest. Measurements of the relative concen tration of cytochromeaa3 did not seem to relate to cerebral FOE. Despite the limitations referred to above, changes recorded by various near infrared spectroscopy instruments have been reported elsewhere, and development of devices that are able to do this are considered a high priority by some authors.24

One objective of this study was to discover whether this non-invasive methodology would be suitable for the study of infants undergoing hypothermia for brain protection in other clinical situations. Hypothermia was associated with a decrease in cerebral FOE and differences were shown during rewarming in patients who underwent circulatory arrest compared with those who had continuous flow bypass. The significance of extreme values of FOE, however, remains to be established and requires further study. The measured normal ranges of FOE using near infrared spectroscopy are wide, making interpretation of individual results difficult, but further study should help to clarify what is a safe range for cerebral FOE in different clinical settings.


We would like to thank Mr R Franks, Mr M Pozzi, Dr P Booker, Dr R Thornington, and Dr B Michalska for their help and permission to study their patients. Financial support was provided by the Newborn Appeal and the Alder Hey Research and Development Fund.


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