Whole blood ionised calcium concentration was measured simultaneously in capillary and arterial blood from neonates being nursed on an intensive care unit using an ion selective electrode. The mean arterio-capillary difference was -0.027 mmol/l (95% confidence intervals -0.041 to -0.012 mmol/l) and the limits of agreement between the two measurements were 0.034 and -0.088 mmol/l. Measurement of ionised calcium in capillary blood is acceptable for clinical purposes.
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