Annotation
Pseudomonas and all that
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Introduction |
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A French military surgeon in 1850 was the first to describe an infection with Pseudomonas aeruginosa when he discovered blue pus in the dressings of wounded soldiers.1 The colour resulted from the secretion by the bacterium of its characteristic pigment pyocyanin. By 1984 more than 102 different species were included in the family Pseudomonadaceae, most of which were plant pathogens or soil saprophytes. All are straight or curved Gram negative rods that are motile by polar flagella. Most are strictly aerobic, can grow in temperatures from 4-43°C and are usually oxidase positive. Application of DNA technologies to microbial taxonomy has led to further divisions within the Pseudomonadaceae. This has resulted in a proliferation of "new" genera and species much to the consternation of clinicians and clinical microbiologists alike.
The taxonomic tool used most frequently is analysis of 16S and 23S
ribosomal RNA (rRNA) cistrons. These regions are relatively well
conserved. For
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