Eubacterial approach to the diagnosis of bacterial infection
a Bristol Royal Hospital for Sick Children,
Bristol, b Department of
Pathology and Microbiology, University of Bristol, Bristol
Correspondence to: Dr B E Ley, Department of Paediatric Oncology, Bristol Royal Hospital for Sick Children, St Michael's Hill, Bristol BS2 8JS.
Accepted 29
April 1997
Computed tomography revealed a frontal empyema in an 8 month
old boy who presented with a prolonged convulsion. Before admission he
had received oral antibiotics and all specimens were negative on
culture. The presence of bacteria in cerebrospinal and empyema fluid
was demonstrated using the polymerase chain reaction (PCR) with 16S
rRNA gene primers. A presumptive identification of Streptococcus pneumoniae was made by comparison of PCR products with those
derived from known bacteria using denaturing gradient gel electrophoresis.
© 1997 by Archives of Disease in Childhood
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